Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT . Anti-Human LIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hLIGHT by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human LIGHT (60-206BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 2.5 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 buffer is recommended. Optimal concentrations and conditions may vary. <br /> <br /> <span style=font-size: 10px,><em>Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. <br /> Information and photo are courtesy of Flagship Biosciences, LLC - Histology Services. </em>. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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