Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Anti-Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hMEC by sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human MEC (60-218BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.. Immunohistochemistry: This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.<br /> <br /> <span style=font-size: 10px,>*Additional Immunostaining data available. Please contact Tech Support for information.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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