ACTB Antibody - middle region
Artikelnummer:
ASB-ARP40174_P050
- Bilder (20)
| Artikelname: | ACTB Antibody - middle region |
| Artikelnummer: | ASB-ARP40174_P050 |
| Hersteller Artikelnummer: | ARP40174_P050 |
| Alternativnummer: | ASB-ARP40174_P050-25UL,ASB-ARP40174_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Equine, Goat, Human, Mouse, Porcine, Rat, Sheep, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of Human ACTB |
| Alternative Synonym: | BRWS1, PS1TP5BP1 |
| This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
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Rabbit Anti-ACTB Antibody Catalog Number: ARP40174_P050 Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Sample type: 1-8.N2 WT C elegans (60ug)Primary Dilution: 1:2000Secondary Antibody: goat anti-Rabbit HRPSecondary Dilution: 1:2000 |
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IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. |
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Sample Type: Human brain stem cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution: 1:1000Color/Signal Descriptions: ACTB: Red DAPI:BlueGene Name: ACTBSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science |
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WB Suggested Anti-ACTB Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293T |
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Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1.0ug/ml |
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WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 30ug mouse 3T3 ECM extract + blocking peptide Lane 2: 30ug mouse 3T3 ECM extractLane 3: 30ug Echinococcus granulosus extract Primary Antibody Dilution : 1:300 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Anonymous |
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Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5.0 ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 12% |
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Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
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Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in MCF7 |
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Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells |
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Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml |
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Host: Mouse Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml |
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Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml |
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Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
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Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml |
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1. The sample 1 - 8 is used N2 wild type C. elegans. 2. Each lane contain 60 ug of sample and the loading volume is 36 ul. 3. The number 1 sample is a control without any treatment. 4. As I get 50 ug of primary Ab, I diluted with 50 ul of water. I use 5 ul each time in 10 ml of 5% milk in PBST buffer. 5. The secondary antibody for chemiluminescence is Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (111-035-003) from Jacksonimmunoresearch, for the chromogenic detection, I used goat anti-rabbit IgG-Ap (sc-2007) from santa cruz. |
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ACTB Antibody - middle region (ARP40174_P050) in Human using Western Blot |
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