RNASE1 Antibody - middle region

Artikelnummer: ASB-ARP40432_P050
Artikelname: RNASE1 Antibody - middle region
Artikelnummer: ASB-ARP40432_P050
Hersteller Artikelnummer: ARP40432_P050
Alternativnummer: ASB-ARP40432_P050-25UL,ASB-ARP40432_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: Bovine, Equine, Goat, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNASE1
Alternative Synonym: RAC1, RIB1, RNS1
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3-side of pyrimidine bases. It prefers poly(C) as a substr
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 18 kDa
NCBI: 6035
UniProt: P07998
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-RNASE1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human kidney
25 ug of the indicated Mouse whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
RNASE1 antibody - middle region (ARP40432_P050) in Human kidney using Western Blot