ZNF294 Antibody - N-terminal region
Artikelnummer:
ASB-ARP43201_P050
- Bilder (4)
| Artikelname: | ZNF294 Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP43201_P050 |
| Hersteller Artikelnummer: | ARP43201_P050 |
| Alternativnummer: | ASB-ARP43201_P050-25UL,ASB-ARP43201_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human ZNF294 |
| Alternative Synonym: | RNF160, ZNF294, C21orf10, C21orf98 |
| ZNF294 may function as an E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 6-18% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Several isoforms contain the peptide sequence including ~150 kDa and smaller isoforms from 23 to 53 kDa. |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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WB Suggested Anti-ZNF294 Antibody Titration: 5% Milk ELISA Titer: dilution: 1:500 Positive Control: human LCL and mouse brains |
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ZNF294 antibody - N-terminal region (ARP43201_P050) in human LCL , mouse brains using Western Blot |
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