Host: Rabbit Target: SLC2A3 Positive control (+): Human heart (HE) Negative control (-): Human kidney (KI) Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
SLC2A3 antibody - middle region (ARP63350_P050) in Human COLO205 using Western Blot
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