FOLR2 Antibody - C-terminal region
Artikelnummer:
ASB-ARP65891_P050
- Bilder (4)
| Artikelname: | FOLR2 Antibody - C-terminal region |
| Artikelnummer: | ASB-ARP65891_P050 |
| Hersteller Artikelnummer: | ARP65891_P050 |
| Alternativnummer: | ASB-ARP65891_P050-25UL,ASB-ARP65891_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the C-terminal region of Human FOLR2 |
| Alternative Synonym: | FBP, FOLR1, FR-P3, FRbeta, FR-BETA, BETA-HFR, FBP/PL-1 |
| The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. |
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WB Suggested Anti-FOLR2 Antibody Titration: 1.0 ug/ml Positive Control: MCF7 Whole CellFOLR2 is supported by BioGPS gene expression data to be expressed in MCF7 |
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25 ug of the indicated whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/mL. Multiple isoforms of this protein are known and several share the peptide sequence. |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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Western Blot |
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