SPRTN Antibody - C-terminal region
Artikelnummer:
ASB-ARP68994_P050
- Bilder (4)
| Artikelname: | SPRTN Antibody - C-terminal region |
| Artikelnummer: | ASB-ARP68994_P050 |
| Hersteller Artikelnummer: | ARP68994_P050 |
| Alternativnummer: | ASB-ARP68994_P050-25UL,ASB-ARP68994_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Human |
| Immunogen: | The immunogen is a synthetic peptide directed towards the C-terminal region of Human SPRTN |
| Alternative Synonym: | DVC1, PRO4323, spartan, C1orf124 |
| SPRTN is a regulator of UV-induced DNA damage response: acts as a reader of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. SPRTN is recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. It facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. It is also required for localization of DNA polymerase eta (POLH) to sites of UV damage. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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Host: Rabbit Target Name: SPRTN Sample Type: 293T Whole Cell lysates Antibody Dilution: 1.0ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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SPRTN Antibody - C-terminal region (ARP68994_P050) in Human 293T using Western Blot |
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