STAC3 Antibody - C-terminal region

Artikelnummer: ASB-ARP71681_P050
Artikelname: STAC3 Antibody - C-terminal region
Artikelnummer: ASB-ARP71681_P050
Hersteller Artikelnummer: ARP71681_P050
Alternativnummer: ASB-ARP71681_P050-25UL,ASB-ARP71681_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human STAC3
Alternative Synonym: NAM, MYPBB
The function of this protein remains unknown.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 41 kDa
NCBI: 246329
UniProt: Q96MF2
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Host: Rabbit
Target Name: STAC3
Sample Type: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The canonical 41 kDa protein is shown, as well as isoforms at 37 and 20 kDa that also contain the peptide sequence (Isoforms 1, 2 & 3).
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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