Boster Bio Anti-p38 alpha/MAPK14 Antibody Picoband catalog A00176-2. Tested in Flow Cytometry, IP, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunoprecipitation, 0.5-2 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
Immunoprecipitating p38 alpha/MAPK14 in Jurkat whole cell lysate.Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2), Lane 1: Jurkat whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-p38 alpha/MAPK14 antibody in Jurkat whole cell lysate,Lane 3: anti-p38 alpha/MAPK14 antibody (2µg) + Jurkat whole cell lysate (500µg).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p38 alpha/MAPK14 antigen
Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p38 alpha/MAPK14 antigen affinity purified polyclonal antibody (A00176-2) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200)with Tanon 5200 system. A specific band was detected for p38 alpha/MAPK14 at approximately 38 kDa. The expected band size for p38 alpha/MAPK14 is at 41 kDa.
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
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