| CF dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper. CF dyes are Biotiums line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm. |
