This is an Anti-GAPDH Mouse monoclonal Antibody (2B5) suitable for IF, IHC-P, WB. It is developed using the synthetic peptide as the immunogen and is designed for reactivity with Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast samples.
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[2B5]
Puffer:
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Formulierung:
Liquid solution
Anwendungsbeschreibung:
Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400)
Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).
Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
