Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax Human IL-7, Tag Free at 2 ug/ml (100 uL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 4-31 ng/mL.
Biotinylated Human TSLP (R127A, R130A), His, Avitag immobilized at 2 ug/ml (100 uL/well) via precoated 5 ug/ml (100 uL/well) of Human TSLP R, Fc Tag, can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag
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