Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human/Cynomolgus/Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/ml (100 uL/well) can bind Human B7-2, Fc Tag with a linear range of 0.313-5 ug/ml.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/ml (100 uL/well) can bind Human B7-1, Fc Tag with a linear range of 0.625-10 ug/ml.
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