Factor VIII B chain Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB156821
- Bilder (9)
| Artikelname: | Factor VIII B chain Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB156821 |
| Hersteller Artikelnummer: | orb156821 |
| Alternativnummer: | BYT-ORB156821-50,BYT-ORB156821-100,BYT-ORB156821-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human Factor VIII B chain (1101-1200/2351aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AHF, DXS1253E, F8B, F8C, FVIII, HEMA, THPH13, Cf-8, Cf8, FA8_CANLF, F8, Procoagulant component, FA8_HUMAN, Antihemophilic factor (AHF), FA8_MOUSE, FA8_PIG, |
| Factor VIII B chain Rabbit Polyclonal Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2). |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 138 kDa |
| UniProt: | P00451 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | F8 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Factor VIII B chain) Polyclonal Antibody, Unconjugated (orb156821) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-Factor VIII B chain (orb156821) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD. |
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Sample: Siha Cell (Human) Lysate at 40 ug, Primary: Anti-Factor VIII B chain (orb156821) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 138kD, Observed band size: 135kD. |
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IHC-P analysis of human brain using Factor VIII B chain Polyclonal Antibody at 1:200 dilution. |
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Western blot:Siha Cell(Human),Primary:Anti-Factor VIII B chainat 1:300 dilution. |
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