ARID domain-containing protein 1A,AT rich interactive domain 1A (SWI-like),AT-rich interactive domain-containing protein 1A,B120,BAF250,BAF250a,BM029,brain protein 120,BRG1-associated factor 250,BRG1-associated factor 250a,C1orf4,chromatin remodeling factor p250,CSS2,ELD,hELD,hOSA1,MRD14,osa homolog 1,OSA1,OSA1 nuclear protein,P270,SMARCF1,SWI/SNF complex protein p270,SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1,SWI-like protein
Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
ARID1A/BAF250
Application Verdünnung:
Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is rec
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of mouse ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of mouse mIMCD-3 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1A/BAF250 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP, 10% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used for IP at 20 µl/mg lysate.
Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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