Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quelle:
Borrelia burgdorferi
Reinheit:
OspB is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspB and was estimated to be greater than 90% pure.
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
ELISA: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Biological Origin: Borrelia burgdorferi. Application Notes: OspB is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 72.7 kDa for OspB-MBP, (30.3 kDa for OspB and 42.4 for MBP) in size corresponding to OspB by Western blotting in the appropriate cell lysate or extract. Outer surface protein B was tested in SDS-page
Antigen-specific IgG2, IgA1, and ADCD partitioning between compartments differs significantly across disease phenotypes. (A) Fraction of samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients in the serum (dashed line) and joint fluid (solid line) for each antigen. Significant differences in distribution of non-zero measurements between fluids as assessed by a Fishers exact test are denoted as *p < 0.05, **p < 0.01, ***p < 0.001 for refractory (dark blue) and responsive (green) samples after correction for multiple hypothesis testing via Benjamini-Hochburg. (B) Ratio of fraction of serum samples with non-zero measurements to fraction of joint fluid samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients for each antigen. Significant differences in distributions of ratios between phenotypes are assessed by a Mann-Whitney nonparametric test, then corrected for multiple hypothesis testing via Benjamini-Hochburg. CRASP1, CRASP2, DbpA, DbpB, Arp37, flagellin, OspA, OspB, OspC, OspE, p27, p35, p39, VlsE: Biorbyt antigens.
SDS PAGE Results of OspB Control Protein. Lane 1: OspB Control Protein Reduced [1 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: OspB Control Protein Non-Reduced [1 µg]. 4-20% Gel, Coomassie Stained.
Systems serology profiling with Borrelia-specific antigens reveals patient heterogeneity. The heatmap shows the Z-scored measurements for 12 features, across 16 antigens for both refractory and responsive patients, visualized with joint fluid measurements in the upper half of the heatmap and serum measurements in the lower half of the heatmap. Only antigens detected above background for at least 30% of samples were included for each measurement. Statistical significance was assessed using the Mann-Whitney nonparametric test, with p values then corrected for multiple hypothesis testing via Benjamini-Hochburg, *p < 0.05, **p < 0.01, ***p < 0.001, else not significant. CRASP1 (p/n orb345971), CRASP2 (p/n orb345972), DbpA (p/n orb345961), DbpB (p/n orb345969), Arp37 (p/n orb345962), flagellin (p/n orb345967), OspA (p/n orb345966), OspB (p/n orb345968), OspC (p/n orb345964), OspE (p/n orb345963), p27 (p/n orb345973), p35 (p/n orb345965), p39 (p/n orb345970), VlsE (p/n orb345974).
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