This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.
Formulierung:
Lyophilized
Anwendungsbeschreibung:
Biological Origin: Human. Application Notes: Human Transferrin Biotin has been tested in dot blot and is designed for Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates
Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 60 min at RT.
Dot Blot results of Human Transferrin Biotin Conjugated. Antigen: Human Transferrin Biotin Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.
Ex vivo fluorescence imaging of biodistribution of the nanoparticles in an orthotopic mouse model of PBT. Notes: (A) Fluorescent signal observed in the brains of four separate mice with PBT (1-24 hours) injected intravenously (via tail vein) with transferrin-coated Prussian blue nanoparticles (MnPB-ATxRd-Tf). A control mouse with PBT was not injected with nanoparticles. (B) Representative ex vivo fluorescence imaging of organ biodistribution of the nanoparticles at 3 hours post-injection. (C) Histograms quantifying the observed fluorescence biodistribution of the nanoparticles 3 hours post-injection. ROIs for intensity measurements are indicated by white dashed lines in (B). Abbreviations: PBT, pediatric brain tumor, MnPB, manganese-containing Prussian blue, ATxRd, Texas Red-labeled avidin, Tf, transferrin, ROI, region of interest, au, arbitrary units. Human Transferrin Biotin Conjugated (p/n orb346261).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
