INOS Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB501000
- Bilder (8)
| Artikelname: | INOS Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB501000 |
| Hersteller Artikelnummer: | orb501000 |
| Alternativnummer: | BYT-ORB501000-50,BYT-ORB501000-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human NOS-2 (101-200/1153aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HEP-NOS, INOS, NOS, NOS2A, MAC-NOS, NOS-II, Nos-2, i-NOS, NOS2_HUMAN, NOS2, Hepatocyte NOS (HEP-NOS), Inducible NO synthase (Inducible NOS | iNOS), NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, 1.14.13.39, NOS2_MOUSE, Macrophage NOS (MAC-NOS), Inosl, NOS2_RAT, |
| INOS Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (orb501000) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (orb501000) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Mouse Raw264.7 tissue lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-iNOS (orb501000) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kDa, Observed band size: 140 kDa. |
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Sample: U251 Cell Lysate at 40 ug, Primary: Anti-iNOS (orb501000) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 130kD, Observed band size: 130kD. |
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Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (orb501000) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated (orb501000) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Western blot:U251 Cell,Primary:Anti-iNOSat 1:300 dilution. |
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Western blot:Lane 1:Mouse Raw264.7,Lane 2:Human Jurkat cell,Primary:Anti-iNOS at 1:1000 dilution. |
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