Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody, Clone: [7A12], Unconjugated
Artikelnummer:
BYT-ORB559128
- Bilder (8)
| Artikelname: | Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody, Clone: [7A12], Unconjugated |
| Artikelnummer: | BYT-ORB559128 |
| Hersteller Artikelnummer: | orb559128 |
| Alternativnummer: | BYT-ORB559128-50,BYT-ORB559128-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Ser380 GF(p-S)FV |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RPS6KA1 | p90-RSK1 (p-S380), p-p90-RSK1, phospho-p90-RSK1, KS6A1_MOUSE, Rps6ka1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1 | p90RSK1 | p90S6K), MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a), Ribosomal S6 kinase 1 (RSK-1), 2.7.11.1, Mapkapk1a, Rsk1, KS6A1_RAT, HU-1, MAPKAPK1, RSK, p90Rsk, |
| Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [7A12] |
| Molekulargewicht: | 90 kDa |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | RPS6KA1 |
| Application Verdünnung: | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: A431 cell lysates, Primary: Anti-Phospho-RPS6KA1 (Ser380) (orb559128) at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD. |
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IHC-P analysis of rat testis using Phospho-RPS6KA1 Ser380 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of human colon using Phospho-RPS6KA1 Ser380 Monoclonal Antibody at 1:200 dilution. |
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