ACAA2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA001118LA01HU
- Bilder (4)
| Artikelname: | ACAA2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA001118LA01HU |
| Hersteller Artikelnummer: | CSB-PA001118LA01HU |
| Alternativnummer: | CSB-PA001118LA01HU-100UG, CSB-PA001118LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Konjugation: | Unconjugated |
| Alternative Synonym: | 3 ketoacyl CoA thiolase antibody, 3 ketoacyl CoA thiolase mitochondrial antibody, 3-ketoacyl-CoA thiolase, mitochondrial antibody, 3-oxoacyl-CoA thiolase, mitochondrial antibody, ACAA 2 antibody, Acaa2 antibody, Acetyl CoA acyltransferase 2 antibody, Acetyl CoA acyltransferase antibody, Acetyl Coenzyme A acyltransferase 2 antibody, Acetyl-CoA acyltransferase antibody, Beta ketothiolase antibody, Beta-ketothiolase antibody, DSAEC antibody, Mitochondrial 3 oxoacyl CoA thiolase antibody, Mitochondrial 3 oxoacyl Coenzyme A thiolase antibody, Mitochondrial 3-oxoacyl-CoA thiolase antibody, T1 antibody, THIM_HUMAN antibody |
| Klonalität: | Polyclonal |
| UniProt: | P42765 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | ACAA2 |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:500, IP:1:200-1:2000 |
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Immunofluorescent analysis of HepG2 cells using CSB-PA001118LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue All lanes: ACAA2 antibody at 2.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa |
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IHC image of CSB-PA001118LA01HU diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating ACAA2 in A549 whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA001118LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA001118LA01HU (6ug) + A549 whole cell lysate (500ug) Lane 3: A549 whole cell lysate (10ug) |
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