ALDH3B1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA001574LA01HU
Artikelname: ALDH3B1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA001574LA01HU
Hersteller Artikelnummer: CSB-PA001574LA01HU
Alternativnummer: CSB-PA001574LA01HU-100UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, WB
Spezies Reaktivität: Human, Mouse
Konjugation: Unconjugated
Alternative Synonym: ALDH3B1 antibody, ALDH7Aldehyde dehydrogenase family 3 member B1 antibody, EC 1.2.1.28 antibody, EC 1.2.1.5 antibody, EC 1.2.1.7 antibody, Aldehyde dehydrogenase 7 antibody
Klonalität: Polyclonal
UniProt: P43353
Puffer: Preservative: 0.02% sodium azide<br />Constituents: PBS containing 50% glycerol pH 7.3
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: ALDH3B1
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
IHC image of CSB-PA001574LA01HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA001574LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in:RAW264.7 whole cell lysate, MCF7 whole cell lysate, K549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate
All lanes:ALDH3B1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52kDa
Observed band size: 52 kDa