CSTB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA00224A0RB
- Bilder (5)
| Artikelname: | CSTB Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA00224A0RB |
| Hersteller Artikelnummer: | CSB-PA00224A0Rb |
| Alternativnummer: | CSB-PA00224A0RB-100UG, CSB-PA00224A0RB-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CHROW21 antibody, CPI B antibody, CPI-B antibody, CST 6 antibody, CST6 antibody, CSTB antibody, Cystatin B (stefin B) antibody, Cystatin B antibody, Cystatin-B antibody, CYTB antibody, CYTB_HUMAN antibody, EPM1 antibody, EPM1A antibody, Liver thiol proteinase inhibitor antibody, PME antibody, Stefin-B antibody, STF B antibody, STFB antibody, ULD antibody |
| Klonalität: | Polyclonal |
| UniProt: | P04080 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | CSTB |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00224A0Rb at dilution of 1:100 |
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IHC image of CSB-PA00224A0Rb diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunofluorescence staining of HepG2 cells with CSB-PA00224A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunoprecipitating CSTB in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA00224A0Rb in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA00224A0Rb (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg) |
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Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate All lanes: CSTB antibody at 5.36µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa |
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