BCL2L1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA002613HA01HU
Artikelname: BCL2L1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA002613HA01HU
Hersteller Artikelnummer: CSB-PA002613HA01HU
Alternativnummer: CSB-PA002613HA01HU-100UG, CSB-PA002613HA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human, Mouse
Konjugation: Unconjugated
Alternative Synonym: Apoptosis regulator Bcl X antibody, Apoptosis regulator Bcl-X antibody, Apoptosis regulator BclX antibody, B cell lymphoma 2 like antibody, B2CL1_HUMAN antibody, Bcl 2 like 1 protein antibody, Bcl X antibody, Bcl xL antibody, BCL XL/S antibody, Bcl xS antibody, Bcl-2-like protein 1 antibody, Bcl2 Like 1 antibody, Bcl2 related gene antibody, Bcl2-L-1 antibody, BCL2L antibody, Bcl2l1 antibody, BCLX antibody, BclXL antibody, BclXs antibody, DKFZp781P2092 antibody, PPP1R52 antibody, Protein phosphatase 1 regulatory subunit 52 antibody
Klonalität: Polyclonal
UniProt: Q07817
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: BCL2L1
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Western Blot
Positive WB detected in: RAW264.7 whole cell lysate
All lanes: BCL2L1 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 19, 26 kDa
Observed band size: 27 kDa
Immunofluorescence staining of MCF-7 cells with CSB-PA002613HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA002613HA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA002613HA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.