EIF4A2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA00384A0RB
Artikelname: EIF4A2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA00384A0RB
Hersteller Artikelnummer: CSB-PA00384A0Rb
Alternativnummer: CSB-PA00384A0RB-100UG, CSB-PA00384A0RB-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: ATP dependent RNA helicase eIF4A 2 antibody, ATP-dependent RNA helicase eIF4A-2 antibody, BM-010 antibody, DDX2B antibody, eIF 4A II antibody, eIF-4A-II antibody, EIF4A antibody, eIF4A II antibody, eIF4A-II antibody, EIF4A2 antibody, EIF4F antibody, Eukaryotic initiation factor 4A II antibody, Eukaryotic initiation factor 4A-II antibody, eukaryotic translation initiation factor 4A isoform 2 antibody, Eukaryotic translation initiation factor 4A2 antibody, IF4A2_HUMAN antibody, N-terminally processed antibody
Klonalität: Polyclonal
UniProt: Q14240
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: EIF4A2
Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
All lanes: EIF4A2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa