CAPN2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA004496LA01HU
- Bilder (5)
| Artikelname: | CAPN2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA004496LA01HU |
| Hersteller Artikelnummer: | CSB-PA004496LA01HU |
| Alternativnummer: | CSB-PA004496LA01HU-100UG, CSB-PA004496LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Calcium activated neutral proteinase 2 antibody, Calcium activated neutral proteinase antibody, Calcium-activated neutral protease 2, catalytic subunit antibody, Calcium-activated neutral proteinase 2 antibody, CALP80 antibody, Calpain 2 (m/II) large subunit antibody, Calpain 2 catalytic subunit antibody, Calpain 2 large catalytic subunit antibody, Calpain 2 large subunit antibody, Calpain 2, large [catalytic] subunit antibody, Calpain large polypeptide L2 antibody, Calpain M type antibody, Calpain M-type antibody, Calpain-2 catalytic subunit antibody, Calpain-2 large subunit antibody, Calpain2 antibody, CAN2_HUMAN antibody, CANP 2 antibody, CANP L2 antibody, CANP2 antibody, CANPL 2 antibody, CANPL2 antibody, CANPml antibody, Capa2 antibody, CAPN 2 antibody, CAPN2 antibody, FLJ39928 antibody, M calpain antibody, M calpin antibody, M type antibody, M-calpain antibody, mCANP antibody, Millimolar calpain antibody, Millimolar-calpain antibody |
| Klonalität: | Polyclonal |
| UniProt: | P17655 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | CAPN2 |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
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Immunofluorescence staining of HepG2 cells with CSB-PA004496LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA004496LA01HU diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA004496LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating CAPN2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA004496LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA004496LA01HU (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg) |
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Western Blot Positive WB detected in: 293T whole cell lysate All lanes: CAPN2 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 72 kDa Observed band size: 80 kDa |
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