Phospho-DNA PKcs (S2056) (5N13) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE05886
Produktübersicht
Artikelname:
Phospho-DNA PKcs (S2056) (5N13) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE05886
Hersteller Artikelnummer:
AMRe05886
Alternativnummer:
EKL-AMRE05886-50UL, EKL-AMRE05886-100UL, EKL-AMRE05886-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ICC, IHC, WB
Spezies Reaktivität:
Human
Konjugation:
Unconjugated
Alternative Synonym:
DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK
Produktbeschreibung
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRKDC must be bound to DNA to express its catalytic properties. Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage (PubMed:11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (PubMed: 11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Must be bound to DNA to express its catalytic properties (PubMed: 11955432). Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) (PubMed: 11955432). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805, PubMed: 33854234). Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription (PubMed: 15574326, PubMed: 11955432, PubMed: 12649176, PubMed: 14734805). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit proc
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
469kDa
NCBI:
5591
UniProt:
P78527
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
PRKDC
Anwendungsbeschreibung
Application Verdünnung:
WB 1:1000-1:2000,IHC 1:50-1:200,ICC/IF 1:20-1:100
