Phospho-IRF3 (S386) (7S3) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE05927
| Artikelname: |
Phospho-IRF3 (S386) (7S3) Rabbit Monoclonal Antibody, Unconjugated |
| Artikelnummer: |
EKL-AMRE05927 |
| Hersteller Artikelnummer: |
AMRe05927 |
| Alternativnummer: |
EKL-AMRE05927-50UL, EKL-AMRE05927-100UL, EKL-AMRE05927-200UL |
| Hersteller: |
EnkiLife |
| Wirt: |
Rabbit |
| Kategorie: |
Antikörper |
| Applikation: |
ICC, WB |
| Spezies Reaktivität: |
Human |
| Konjugation: |
Unconjugated |
| Alternative Synonym: |
IRF3, Interferon regulatory factor 3, |
| Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. Key transcriptional regulator of type I interferon (IFN)- dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148). |
| Klonalität: |
Monoclonal |
| Molekulargewicht: |
47kDa |
| NCBI: |
3661 |
| UniProt: |
Q14653 |
| Puffer: |
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA. |
| Reinheit: |
Affinity purification |
| Formulierung: |
Liquid |
| Target-Kategorie: |
IRF3 |
| Application Verdünnung: |
WB 1:1000-1:2000,ICC/IF 1:50-1:200 |
