Phospho-JAK2 (Y1007 + Y1008) (17F11) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE05930
Produktübersicht
Artikelname:
Phospho-JAK2 (Y1007 + Y1008) (17F11) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE05930
Hersteller Artikelnummer:
AMRe05930
Alternativnummer:
EKL-AMRE05930-50UL, EKL-AMRE05930-100UL, EKL-AMRE05930-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, IHC, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
EC 2.7.10.2, JAK-2, JAK2, Janus kinase 2, kinase Jak2,
Produktbeschreibung
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO), or type II receptors including IFN-alpha, IFN- beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed: 9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed: 21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed: 20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed: 21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of Tyr-41 of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed: 19783980).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
131kDa
NCBI:
3717
UniProt:
O60674
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
JAK2
Anwendungsbeschreibung
Application Verdünnung:
WB 1:1000-1:15000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,FC 1:20-1:50
