OGT (19A13) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE15124
| Artikelname: |
OGT (19A13) Rabbit Monoclonal Antibody, Unconjugated |
| Artikelnummer: |
EKL-AMRE15124 |
| Hersteller Artikelnummer: |
AMRe15124 |
| Alternativnummer: |
EKL-AMRE15124-50UL, EKL-AMRE15124-100UL, EKL-AMRE15124-200UL |
| Hersteller: |
EnkiLife |
| Wirt: |
Rabbit |
| Kategorie: |
Antikörper |
| Applikation: |
FC, ICC, IHC, WB |
| Spezies Reaktivität: |
Human, Mouse, Rat |
| Konjugation: |
Unconjugated |
| Alternative Synonym: |
HRNT1, O-GLCNAC, |
| Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N- acetylglucosamine (O-GlcNAc) (PubMed:26678539, PubMed:23103939, PubMed:21240259, PubMed:21285374, PubMed:15361863). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed:21285374). Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (PubMed:26678539). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the Thr-308 phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of Ser-112 of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of Ser-75 of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O- glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:12150998, PubMed:19451179, PubMed:20018868, PubMed:20200153, PubMed:21285374, PubMed:15361863). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed:21285374, PubMed:28584052, |
| Klonalität: |
Monoclonal |
| Molekulargewicht: |
117kDa |
| NCBI: |
8473 |
| UniProt: |
O15294 |
| Puffer: |
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA. |
| Reinheit: |
Affinity purification |
| Formulierung: |
Liquid |
| Target-Kategorie: |
OGT |
| Application Verdünnung: |
WB 1:500-1:2000,IHC 1:20-1:50,ICC/IF 1:20-1:100,FC 1:20-1:50 |
