PHD3 (11Y3) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE16061
Produktübersicht
Artikelname:
PHD3 (11Y3) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE16061
Hersteller Artikelnummer:
AMRe16061
Alternativnummer:
EKL-AMRE16061-50UL, EKL-AMRE16061-100UL, EKL-AMRE16061-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ICC, IHC, IP, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
Egl nine homolog 3, EGLN3, Factor responsive smooth muscle protein, HIF Prolyl Hydroxylase 3, HIFP4H3, HIFPH3, P4H3, PHD3, SM20,
Produktbeschreibung
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at Pro-564, and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A (PubMed:19584355, PubMed: 21620138, PubMed: 21483450, PubMed: 22797300, PubMed: 20978507, PubMed: 21575608). Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins (PubMed: 11595184, PubMed: 12181324). Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N- terminal, NODD, and C-terminal, CODD) of HIF1A (PubMed: 11595184, PubMed: 12181324). Also hydroxylates HIF2A (PubMed: 11595184, PubMed: 12181324). Has a preference for the CODD site for both HIF1A and HIF2A (PubMed: 11595184, PubMed: 12181324). Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site (PubMed: 11595184, PubMed: 12181324). Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed: 11595184, PubMed: 12181324). Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy- inducible genes (PubMed: 11595184, PubMed: 12181324). ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis (PubMed: 21620138, PubMed: 21483450). Under normoxia, hydroxylates and regulates the stability of ADRB2 (PubMed: 19584355). Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex (PubMed: 20849813). In neurons, has a NGF-induced proapoptotic effect, probably through regula
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
27kDa
NCBI:
112399
UniProt:
Q9H6Z9
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
EGLN3
Anwendungsbeschreibung
Application Verdünnung:
WB 1:500-1:2000,IHC 1:50-1:100,ICC/IF 1:20-1:50,IP 1:20-1:50
