Human HMGA1 (High Mobility Group AT Hook Protein 1) ELISA Kit
Artikelnummer:
EKL-EH25968
Hersteller Artikelnummer:
EH25968
Alternativnummer:
EKL-EH25968-96T
Hersteller:
EnkiLife
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human HMGA1. You can calculate the concentration of Human HMGA1 in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich:
0.31-20ng/mL
Sensitivitaet:
0.115ng/mL
Proben:
Serum,plasma and other biological fluids
Target-Kategorie:
HMGA1
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