Human UBE2I (Ubiquitin Conjugating Enzyme E2I) ELISA Kit

Artikelnummer: EKL-EH26141
Artikelname: Human UBE2I (Ubiquitin Conjugating Enzyme E2I) ELISA Kit
Artikelnummer: EKL-EH26141
Hersteller Artikelnummer: EH26141
Alternativnummer: EKL-EH26141-96T
Hersteller: EnkiLife
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: P18, UBC9, UBCE9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, SUMO-conjugating enzyme UBC9
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UBE2I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE2I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE2I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human UBE2I. You can calculate the concentration of Human UBE2I in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich: 0.15-10ng/mL
Sensitivitaet: 0.057ng/mL
Proben: Serum,plasma and other biological fluids
Target-Kategorie: UBE2I