Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Ala134-Leu285
Target-Kategorie:
BAFF/TNFSF13B/CD257
Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA.
Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BCMA (Trimer), His Tag with an affinity constant of 0.36 nM as determined in BLI assay (Gator Prime).
Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BAFFR, His Tag with an affinity constant of 34.80 nM as determined in BLI assay (Gator Prime).
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