Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
>95% as determined by Bis-Tris PAGE
Formulierung:
Lyophilized
Sequenz:
Pro20-Lys291
Target-Kategorie:
CD19
Human CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 8.5 ng/ml determined by ELISA.
Immobilized Human CD19, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 8.6 ng/ml determined by ELISA.
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.3 ng/ml determined by ELISA (QC Test).
Immobilized Human CD19, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 11.1 ng/ml determined by ELISA (QC Test).
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human CD19, His Tag with an affinity constant of 0.50 nM as determined in SPR assay (Biacore T200).
Loaded Denintuzumab, hFc Tag on ProA-Biosensor, can bind Human CD19, His Tag with an affinity constant of 3.73 nM as determined in BLI assay (Gator).
Loaded Inebilizumab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 8.03 nM as determined in BLI assay (Gator Prime).
Loaded Coltuximab on ProA-Biosensor can bind Human CD19, His Tag with an affinity constant of 5.65 nM as determined in BLI assay (Gator Prime).
Loaded FMC63 on MFc-Biosensor can bind Human CD19, His Tag with an affinity constant of 1.44 nM as determined in BLI assay (Gator Prime).
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