Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus of CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).
Molekulargewicht:
The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Target-Kategorie:
CD3E&CD3G/CD3 epsilon&CD3 gamma
Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.
Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).
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