Recombinant Human CD58 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe29-Arg215.
Molekulargewicht:
The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)
UniProt:
AAH05930
Puffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Phe29-Arg215
Target-Kategorie:
CD58
Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test).
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