Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401B)

Artikelnummer: KAB-CDB-HM401B
Artikelname: Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401B)
Artikelnummer: KAB-CDB-HM401B
Hersteller Artikelnummer: CDB-HM401B
Alternativnummer: KAB-CDB-HM401B-100UG, KAB-CDB-HM401B-500UG
Hersteller: KactusBio
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Biotin
Alternative Synonym: Fc gamma RII, Fc gamma RIIB, Fc gamma RIIC, CD32, CD32b, CD32c, CDw32, FCG2, IGFR2, CD32A, FCG2 , FcGR, FCGR2, FCGR21, FCGR2A, FcgammaRIIB, Fc gammaRIIB, Fcgamma RIIB, Fc gamma RIIB
Recombinant Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217.
Molekulargewicht: The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
Tag: C-His-Avi
UniProt: P31994
Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung: Lyophilized
Sequenz: Ala46-Pro217
Target-Kategorie: Fc gamma RIIB/C (CD32b/c)
The purity of Biotinylated Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB/C, His-Avi Tag with an affinity constant of 3.97 uM as determined in SPR assay (Biacore T200).
Biotinylated Human Fc gamma RIIB/C, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 uM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human Fc gamma RIIB/C, His-Avi Tag on Streptavidin-Biosensor can bind Rituximab with an affinity constant of 2.40 µM as determined in BLI assay (Gator Prime).