Non-biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1)
Artikelnummer:
KAB-COV-VM5S1
Hersteller Artikelnummer:
COV-VM5S1
Alternativnummer:
KAB-COV-VM5S1-100UG, KAB-COV-VM5S1-500UG
Hersteller:
KactusBio
Kategorie:
Proteine/Peptide
Spezies Reaktivität:
Virus
Alternative Synonym:
S1 protein, S glycoprotein Subunit1, S1 protein, Spike protein S1, Spike, sars-cov-2
Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.
Molekulargewicht:
The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)-Avi
UniProt:
QHD43416.1
Puffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Gln14-Arg683
Target-Kategorie:
Spike S1
SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 is greater than 90% as determined by SEC-HPLC.
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA (QC Test).
Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Non-biotinylated Human ACE2, His Tag with an affinity constant of 0.66 nM as determined in BLI assay (Gator Prime).
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