Non-biotinylated Human M-CSF/CSF-1 Protein, Ultra Low Endotoxin (CSF-HM401-UL)

Artikelnummer: KAB-CSF-HM401-UL

Artikelname:	Non-biotinylated Human M-CSF/CSF-1 Protein, Ultra Low Endotoxin (CSF-HM401-UL)
Artikelnummer:	KAB-CSF-HM401-UL
Hersteller Artikelnummer:	CSF-HM401-UL
Alternativnummer:	KAB-CSF-HM401-UL-100UG, KAB-CSF-HM401-UL-500UG
Hersteller:	KactusBio
Kategorie:	Proteine/Peptide
Spezies Reaktivität:	Human
Alternative Synonym:	CSF1, CSF-1, MCSF, M-CSF, MGC31930, Lanimostim

Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu33-Asn190.

Molekulargewicht:	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 28-34 kDa based on Bis-Tris PAGE result.
Tag:	C-His-Avi
UniProt:	P09603
Puffer:	Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:	HEK293
Reinheit:	> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:	Lyophilized
Sequenz:	Glu33-Asn190
Target-Kategorie:	M-CSF/CSF-1

	Human M-CSF on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
	Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL.
	Immobilized Human M-CSF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
	Loaded Human M-CSF, His Tag on Anti-His-Biosensor can bind Human M-CSF R, No Tag with an affinity constant of 0.46 µM as determined in BLI assay (Gator Prime).

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