Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
E.coli
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Asn971-Arg1023
Target-Kategorie:
EGF
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
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