Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE
Formulierung:
Lyophilized
Sequenz:
Leu72-Leu251
Target-Kategorie:
TNFSF15
Biotinylated Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human TNFSF15, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.
Immobilized Mouse DR3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 45.6 ng/ml determined by ELISA (QC Test).
Immobilized Human DR3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 72.2ng/ml determined by ELISA.
Immobilized Biotinylated Human TNFSF15, His Avi Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Biotinylated Human TNFSF15, His-Avi Tag with an affinity constant of 3.13 nM as determined in BLI assay (Gator).
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