Recombinant Human IL-7 R alpha&TSLPR Protein is expressed from HEK293 with His tag at the C-terminus of IL-7 R alpha.It contains Glu21-Asp239 (IL-7 R alpha) and Gln23-Lys231 (TSLPR).
Molekulargewicht:
The protein has a predicted MW of 31.18 kDa (IL-7 R alpha) and 29.03 kDa (TSLPR). Due to glycosylation, the protein migrates to 58-68 kDa (IL-7 R alpha) and 50-58 kDa (TSLPR) based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Glu21-Asp239(IL-7 R alpha) & Gln23-Lys231(TSLPR)
Target-Kategorie:
IL-7 R alpha&TSLPR
The purity of Human IL-7 R alpha&TSLPR is greater than 95% as determined by SEC-HPLC.
Human IL-7 R alpha&TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-7R alpha&TSLPR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLP, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human IL-7R alpha&TSLPR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 15.54 nM as determined in SPR assay (Biacore T200).
Loaded Human TSLP, Fc Tag on ProA-Biosensor can bind IL-7 R alpha&TSLP R, His Tag with an affinity constant of 0.37 nM as determined in BLI assay (Gator Prime).
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