Recombinant Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide.
Molekulargewicht:
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Expression System:
E.coli
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide
Target-Kategorie:
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC)
The purity of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
Loaded Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer on Anti-His-Biosensor can bind Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc-Avi Tag with an affinity constant of 0.65 nM as determined in BLI assay (Gator Prime).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
