Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Phe19-Arg238
Target-Kategorie:
PD-L1/B7-H1
Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.92ng/ml determined by ELISA.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Serial dilutions of Anti-PD-L1 Antibody were added into Human PD-L1, His Tag : Biotinylated Human PD-1, hFc Avi Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 11.7ng/ml.
Loaded Biotinylated Human PD-1, His Tag on Streptavidin-Biosensor can bind Human PD-L1, His Tag with an affinity constant of 0.20 µM as determined in BLI assay (Gator Prime).
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