Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)
Artikelnummer:
KAB-TCC-HM102
Hersteller Artikelnummer:
TCC-HM102
Alternativnummer:
KAB-TCC-HM102-100UG
Hersteller:
KactusBio
Kategorie:
Sonstiges
Spezies Reaktivität:
Human
Konjugation:
Biotin
Alternative Synonym:
KRAS G12D TCR&CD3
Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR.It contains KRAS G12D specific TCR (JD1a41b1), CD3epsilon&CD3gamma, CD3epsilon&CD3delta, and CD3zeta dimers.
Molekulargewicht:
The protein has a predicted MW of 196.0 kDa.
Tag:
C-His
Puffer:
Supplied as 0.22 µm filtered solution in PBS, 200mM L-arginine (pH 7.4). <RED>Notice: Not recommended for flow cytometry.</RED>
Expression System:
HEK293
Formulierung:
Liquid
Sequenz:
KRAS G12D specific TCR (JD1a41b1), CD3epsilon&CD3gamma, CD3epsilon&CD3delta, and CD3zeta dimers
Target-Kategorie:
KRAS G12D TCR&CD3
Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.53ug/ml determined by ELISA.
Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA.
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 0.47 nM as determined in SPR assay (Biacore T200).
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay (Gator Prime).
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 2.60 nM as determined in BLI assay (Gator Prime).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
