Biotinylated Human TSLP Protein (TSP-HM401B)

Artikelnummer: KAB-TSP-HM401B
Artikelname: Biotinylated Human TSLP Protein (TSP-HM401B)
Artikelnummer: KAB-TSP-HM401B
Hersteller Artikelnummer: TSP-HM401B
Alternativnummer: KAB-TSP-HM401B-100UG, KAB-TSP-HM401B-500UG
Hersteller: KactusBio
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Biotin
Alternative Synonym: thymic stromal lymphopoietin, TSLP
Recombinant Biotinylated Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.
Molekulargewicht: The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Tag: C-His-Avi
UniProt: Q969D9
Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung: Lyophilized
Sequenz: Tyr29-Gln159
Target-Kategorie: TSLP
The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.
Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP, His Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator Prime).
Loaded Biotinylated Human TSLP, His Tag on Anti-His-Biosensor can bind Human TSLPR, hFc Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator Prime).