Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)

Artikelnummer: KAB-TSP-HM402B
Artikelname: Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)
Artikelnummer: KAB-TSP-HM402B
Hersteller Artikelnummer: TSP-HM402B
Alternativnummer: KAB-TSP-HM402B-100UG, KAB-TSP-HM402B-500UG
Hersteller: KactusBio
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Biotin
Alternative Synonym: Thymic stromal lymphopoietin, TSLP
Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).
Molekulargewicht: The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
Tag: C-His-Avi
UniProt: Q969D9
Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung: Lyophilized
Sequenz: Tyr29-Gln159(R127A,R130A)
Target-Kategorie: TSLP
Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator Prime).
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 4.87 nM as determined in BLI assay (Gator Prime).