Non-biotinylated Human VEGF165 Protein (VEG-HM465)
Artikelnummer:
KAB-VEG-HM465
Hersteller Artikelnummer:
VEG-HM465
Alternativnummer:
KAB-VEG-HM465-100UG, KAB-VEG-HM465-500UG
Hersteller:
KactusBio
Kategorie:
Proteine/Peptide
Spezies Reaktivität:
Human
Alternative Synonym:
VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,VEGF-165
Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.
Molekulargewicht:
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE
Formulierung:
Lyophilized
Sequenz:
Ala27-Arg191
Target-Kategorie:
VEGF165
Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay (Gator Prime).
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay (Gator Prime).
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay (Gator Prime).
Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody2, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
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