VEGF, VEGFA, VEGF165a, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,VEGF-165
Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.
Molekulargewicht:
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 90% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Ala27-Arg191
Target-Kategorie:
VEGF165
Biotinylated VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 90%.
Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA.
Immobilized Human VEGF R2, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 12.6ng/ml determined by ELISA.
Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).
Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA.
The purity of Biotinylated Human VEGF165 is greater than 90% as determined by SEC-HPLC.
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